TGN-073 is a stimulator of aquaporin 4 (AQP4), an astrocyte water channel. TGN-073 has been shown to increase glymphatic transport in the rat brain by up to 41%.

Physicochemical Properties

Molecular Formula	C18H16N2O3S
Molecular Weight	340.40
CAS #	877459-36-4
PubChem CID	4338178
Appearance	Solid powder
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	6
Heavy Atom Count	24
Complexity	465
Defined Atom Stereocenter Count	0
SMILES	C1(COC2=CC=CN=C2NS(C2=CC=CC=C2)(=O)=O)=CC=CC=C1
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. The effect of a novel AQP4 facilitator, TGN-073, on glymphatic transport captured by diffusion MRI and DCE-MRI. PLoS One. 2023 Mar 15;18(3):e0282955.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~293.77 mM; with ultrasonication (<60°C))

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.34 mM)(saturation unknown) in 10% DMSO 40% PEG300 5% Tween-80 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300, mix well; then add 50 μL Tween-80 to the above system, mix well; then continue to add 450 μL of normal saline to make up to 1 mL. Preparation of normal saline: Dissolve 0.9 g of sodium chloride in ddH₂O and make up to 100 mL to obtain a clear and transparent normal saline solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.34 mM)(saturation unknown) in 10% DMSO 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9377 mL	14.6886 mL	29.3772 mL
	5 mM	0.5875 mL	2.9377 mL	5.8754 mL
	10 mM	0.2938 mL	1.4689 mL	2.9377 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.