TC-F2 is a reversible, non-covalent/irreversible inhibitor of fatty acid amide hydrolase (FAAH) with IC50 of 28 nM. FAAH has been implicated in many human diseases, like cancer, pain, and inflammation, as well as neurological, metabolic, and cardiovascular diseases.

Physicochemical Properties

Molecular Formula	C26H25N5O2
Molecular Weight	439.509005308151
Exact Mass	439.201
CAS #	1304778-15-1
PubChem CID	25198728
Appearance	White to off-white solid powder
LogP	4.932
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	4
Heavy Atom Count	33
Complexity	706
Defined Atom Stereocenter Count	1
SMILES	CCN1C2=CC=CC=C2N(C1=O)[C@H]3CCCN(C3)C4=NC=CC(=N4)C5=CC6=CC=CC=C6O5
InChi Key	NXTBLPPTZRPJCA-IBGZPJMESA-N
InChi Code	InChI=1S/C26H25N5O2/c1-2-30-21-10-4-5-11-22(21)31(26(30)32)19-9-7-15-29(17-19)25-27-14-13-20(28-25)24-16-18-8-3-6-12-23(18)33-24/h3-6,8,10-14,16,19H,2,7,9,15,17H2,1H3/t19-/m0/s1
Chemical Name	1-[(3S)-1-[4-(1-benzofuran-2-yl)pyrimidin-2-yl]piperidin-3-yl]-3-ethylbenzimidazol-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Characterization of fatty acid amide hydrolase activity by a fluorescence-based assay. Anal Biochem. 2018 Apr 1;546:50-57.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~568.82 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.08 mg/mL (4.73 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2753 mL	11.3763 mL	22.7526 mL
	5 mM	0.4551 mL	2.2753 mL	4.5505 mL
	10 mM	0.2275 mL	1.1376 mL	2.2753 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.