TAT TFA (YGRKKRRQRRR), developed from the transactivator of transcription (TAT) of human immunodeficiency virus (HIV-1), is a cell-penetrable peptide. TAT can increase the yield and solubility of heterologous proteins.

Physicochemical Properties

Molecular Formula	C??H???N??F?O??
Molecular Weight	1673.85
Related CAS #	TAT;191936-91-1
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	TAT (YGRKKRRQRRR) denotes amino acids 47–57 of the HIV-1 TAT protein. The positively charged protein transduction domain of the HIV-1 TAT protein (TAT-PTD; TAT residues 47–57) quickly translocates across the plasma membrane of live cells. TAT (YGRKKRRQRRR; TAT-PTD) can traverse the hydrophobic lipid bilayer of a cell membrane via passive diffusion[1].
References	[1]. Interaction of the protein transduction domain of HIV-1 TAT with heparan sulfate: binding mechanism and thermodynamic parameters. Biophys J. 2004 Jan;86(1 Pt 1):254-63.

Solubility Data

Solubility (In Vitro)	May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (59.74 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.5974 mL	2.9871 mL	5.9743 mL
	5 mM	0.1195 mL	0.5974 mL	1.1949 mL
	10 mM	0.0597 mL	0.2987 mL	0.5974 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.