Sulfo-Cy7.5 NHS ester is a near-infrared water-soluble (H2O-soluble) hydrophilic dye and an NHS ester used for amine modification. Sulfo-Cy7.5 NHS ester contains a trimethylalkyne bridge and has a linker arm for its attachment to proteins, peptides and other molecules. Sulfo-Cy7.5 NHS ester can be used for research in near-infrared imaging applications.

Physicochemical Properties

Molecular Formula	C49H52KN3O16S4
Molecular Weight	1106.30599021912
Exact Mass	1103.171
CAS #	2736437-44-6
PubChem CID	171975657
Appearance	Light green to green solid powder
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	17
Rotatable Bond Count	13
Heavy Atom Count	73
Complexity	2770
Defined Atom Stereocenter Count	0
SMILES	CC1(C(=[N+](CCCCCC(=O)ON2C(CCC2=O)=O)C2C=CC3=C(C=C(S([O-])(=O)=O)C=C3C1=2)S(O)(=O)=O)C=CC1CCCC(=CC=C2N(C3C=CC4=C(C=C(S(O)(=O)=O)C=C4C=3C2(C)C)S(O)(=O)=O)C)C=1)C.[KH]
InChi Key	HDBJLDCQQJVKRA-UHFFFAOYSA-M
InChi Code	InChI=1S/C49H51N3O16S4.K/c1-48(2)41(50(5)37-17-15-33-35(46(37)48)25-31(69(56,57)58)27-39(33)71(62,63)64)19-13-29-10-9-11-30(24-29)14-20-42-49(3,4)47-36-26-32(70(59,60)61)28-40(72(65,66)67)34(36)16-18-38(47)51(42)23-8-6-7-12-45(55)68-52-43(53)21-22-44(52)54;/h13-20,24-28H,6-12,21-23H2,1-5H3,(H3-,56,57,58,59,60,61,62,63,64,65,66,67);/q;+1/p-1
Chemical Name	potassium;(2Z)-3-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-1,1-dimethyl-2-[(2Z)-2-[3-[(E)-2-(1,1,3-trimethyl-6,8-disulfobenzo[e]indol-3-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]benzo[e]indole-6,8-disulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Ultrafast, Ultrasensitive Detection and Imaging of Single Cardiac Troponin-T Molecules. ACS Sens. 2020 Nov 25;5(11):3576-3583.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~45.28 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (1.13 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (1.13 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.9039 mL	4.5195 mL	9.0391 mL
	5 mM	0.1808 mL	0.9039 mL	1.8078 mL
	10 mM	0.0904 mL	0.4520 mL	0.9039 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.