Sulfo-CY5-NHS ester (potassium) is a reactive dye that may be utilized to label proteins and nucleic acids and is water-soluble (H2O-soluble).

Physicochemical Properties

Molecular Formula	C36H40N3NAO10S2
Molecular Weight	761.8367
Exact Mass	761.21
Elemental Analysis	C, 56.76; H, 5.29; N, 5.52; Na, 3.02; O, 21.00; S, 8.42
CAS #	2230212-27-6
Related CAS #	2230212-27-6 (sodium); 937739-58-7 (inner salt)
PubChem CID	118987356
Appearance	Solid powder
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	11
Rotatable Bond Count	11
Heavy Atom Count	52
Complexity	1690
Defined Atom Stereocenter Count	0
InChi Key	JYLBFAAZKFROET-UHFFFAOYSA-M
InChi Code	InChI=1S/C36H41N3O10S2.Na/c1-35(2)26-22-24(50(43,44)45)15-17-28(26)37(5)30(35)12-8-6-9-13-31-36(3,4)27-23-25(51(46,47)48)16-18-29(27)38(31)21-11-7-10-14-34(42)49-39-32(40)19-20-33(39)41;/h6,8-9,12-13,15-18,22-23H,7,10-11,14,19-21H2,1-5H3,(H-,43,44,45,46,47,48);/q;+1/p-1
Chemical Name	sodium;1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate
Synonyms	Sulfo-Cyanine5 NHS Ester; Sulfo-Cy5-NHS Ester
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Antibody Response against Cowpea Mosaic Viral Nanoparticles Improves In Situ Vaccine Efficacy in Ovarian Cancer. ACS Nano. 2020;14(3):2994-3003.

Solubility Data

Solubility (In Vitro)

DMSO : ~62.5 mg/mL (~80.3 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (2.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.3126 mL	6.5631 mL	13.1261 mL
	5 mM	0.2625 mL	1.3126 mL	2.6252 mL
	10 mM	0.1313 mL	0.6563 mL	1.3126 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.