Succinic anhydride is a cyclic anhydride and non-cleavable (non-degradable) ADC linker. For more details, check and find patent WO2009064913A1. Succinic anhydride reacts with the patented compound 4 to link the precursor to the amine or hydroxyl group of the targeting peptide1.

Physicochemical Properties

Molecular Formula	C4H4O3
Molecular Weight	100.0728
Exact Mass	100.016
CAS #	108-30-5
Related CAS #	Succinic anhydride-d4;14341-86-7;Succinic anhydride-13C2;67519-25-9
PubChem CID	7922
Appearance	White to light yellow solid powder
Density	1.4±0.1 g/cm3
Boiling Point	261.0±0.0 °C at 760 mmHg
Melting Point	118-120 °C(lit.)
Flash Point	122.1±15.9 °C
Vapour Pressure	0.0±0.5 mmHg at 25°C
Index of Refraction	1.470
LogP	-1.3
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	7
Complexity	102
Defined Atom Stereocenter Count	0
InChi Key	RINCXYDBBGOEEQ-UHFFFAOYSA-N
InChi Code	InChI=1S/C4H4O3/c5-3-1-2-4(6)7-3/h1-2H2
Chemical Name	oxolane-2,5-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Chimer containing a targeting portion linked to a scission-activated duocarmycin-type prodrug. WO2009064913A1.

Additional Infomation

Succinic anhydride appears as colorless needles or white crystalline solid. Melting point 237 °F. Sublimes at 239 °F at 5 mmHg pressure; and at 198 °F and 1 mmHg pressure. Moderately toxic and an irritant. Succinic anhydride is a cyclic dicarboxylic anhydride and a tetrahydrofurandione. It is functionally related to a succinic acid. Succinic anhydride has been reported in Clerodendrum japonicum, Nicotiana tabacum, and other organisms with data available. See also: ... View More ...

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~999.30 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (24.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (24.98 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (24.98 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	9.9930 mL	49.9650 mL	99.9300 mL
	5 mM	1.9986 mL	9.9930 mL	19.9860 mL
	10 mM	0.9993 mL	4.9965 mL	9.9930 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.