Stachyose tetrahydrate, a functional oligosaccharide, works as a prebiotic. Stachyose tetrahydrate may indirectly prevent the growth of colon cancer/tumor cells by promoting the proliferation of probiotic bacteria or the production of beneficial substances in the intestines.

Physicochemical Properties

Molecular Formula	C24H42O21.4(H2O)
Molecular Weight	738.65
Exact Mass	738.264
CAS #	10094-58-3
Related CAS #	Stachyose hydrate;54261-98-2;Stachyose;470-55-3
PubChem CID	2724335
Appearance	White to yellow solid powder
Boiling Point	1044.2ºC at 760 mmHg
Melting Point	95-105 °C
Flash Point	585.3ºC
Vapour Pressure	0mmHg at 25°C
Index of Refraction	133 ° (C=5, H2O)
LogP	-8
Hydrogen Bond Donor Count	18
Hydrogen Bond Acceptor Count	25
Rotatable Bond Count	11
Heavy Atom Count	49
Complexity	933
Defined Atom Stereocenter Count	19
SMILES	C([C@@H]1[C@@H]([C@@H]([C@H]([C@H](O1)OC[C@@H]2[C@@H]([C@@H]([C@H]([C@H](O2)OC[C@@H]3[C@H]([C@@H]([C@H]([C@H](O3)O[C@]4([C@H]([C@@H]([C@H](O4)CO)O)O)CO)O)O)O)O)O)O)O)O)O)O
InChi Key	UQZIYBXSHAGNOE-XNSRJBNMSA-N
InChi Code	InChI=1S/C24H42O21/c25-1-6-10(28)14(32)17(35)21(41-6)39-3-8-11(29)15(33)18(36)22(42-8)40-4-9-12(30)16(34)19(37)23(43-9)45-24(5-27)20(38)13(31)7(2-26)44-24/h6-23,25-38H,1-5H2/t6-,7-,8-,9-,10+,11+,12-,13-,14+,15+,16+,17-,18-,19-,20+,21+,22+,23-,24+/m1/s1
Chemical Name	(2S,3R,4S,5R,6R)-2-[[(2R,3R,4S,5R,6S)-6-[[(2R,3S,4S,5R,6R)-6-[(2S,3S,4S,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methoxy]-3,4,5-trihydroxyoxan-2-yl]methoxy]-6-(hydroxymethyl)oxane-3,4,5-triol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Stachyose tetrawater causes apoptosis and inhibits Caco-2 cell division in a dose-dependent way [1]. By encouraging lactic acid bacteria (LAB) to create more α-galactosidase to hydrolyze stachyose, stachyose greatly enhances LAB growth [2].
References	[1]. Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway. Food Funct. 2015;6(3):765-771. [2]. The Proliferation Mechanism of Lactobacillus plantarum RB1 Stimulated by Stachyose. Curr Microbiol. 2017 Jun;74(6):732-738.
Additional Infomation	Stachyose tetrahydrate is an oligosaccharide.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~338.46 mM) H2O : ~100 mg/mL (~135.38 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (2.82 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (2.82 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (2.82 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 100 mg/mL (135.38 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.3538 mL	6.7691 mL	13.5382 mL
	5 mM	0.2708 mL	1.3538 mL	2.7076 mL
	10 mM	0.1354 mL	0.6769 mL	1.3538 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.