Spiculisporic acid is a biologically active gamma-butenolide extracted from marine Aspergillus. Spiculisporic acid is a microbial biosurfactant with antioxidant effects.

Physicochemical Properties

Molecular Formula	C17H28O6
Molecular Weight	328.40
Exact Mass	328.189
CAS #	469-77-2
PubChem CID	316426
Appearance	White to off-white solid powder
Density	1.163g/cm3
Boiling Point	549ºC at 760mmHg
Melting Point	145ºC
Flash Point	195.4ºC
Vapour Pressure	1.68E-13mmHg at 25°C
Index of Refraction	1.502
LogP	3.378
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	12
Heavy Atom Count	23
Complexity	419
Defined Atom Stereocenter Count	0
InChi Key	TUXHHVJPGQUPCF-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H28O6/c1-2-3-4-5-6-7-8-9-10-13(15(19)20)17(16(21)22)12-11-14(18)23-17/h13H,2-12H2,1H3,(H,19,20)(H,21,22)
Chemical Name	2-(1-carboxyundecyl)-5-oxooxolane-2-carboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	A microbial biosurfactant, speficisporic acid is a special long-chain fatty acid that has two carboxylic moieties and a lactonering. By using the yeast DNA microarray bioassay, sporicisporic acid has been shown to be nontoxic in the environment and to have anti-oxidative stress properties in biolocal systems[2].
References	[1]. Spiculisporic acids B–D, three new γ-butenolide derivatives from a sea urchin-derived fungus Aspergillus sp. HDf2. Molecules. 2012 Nov 5;17(11):13175-82. [2]. Molecular and crystal structure of spiculisporic acid and correlation with the surface activity. J Oleo Sci. 2013;62(10):795-802.

Solubility Data

Solubility (In Vitro)

DMSO: 250 mg/mL (761.27 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (6.33 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.33 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (6.33 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0451 mL	15.2253 mL	30.4507 mL
	5 mM	0.6090 mL	3.0451 mL	6.0901 mL
	10 mM	0.3045 mL	1.5225 mL	3.0451 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.