Spermine NONOate is a complex of nitric oxide (NO) and spermine that serves as a NO donor. Spermine NONOate can be used for the preparation of NO.

Physicochemical Properties

Molecular Formula	C10H26N6O2
Molecular Weight	262.35
Exact Mass	262.211
CAS #	136587-13-8
PubChem CID	135412725
Appearance	White to off-white solid powder
Density	1.2±0.1 g/cm3
Boiling Point	425.4±55.0 °C at 760 mmHg
Melting Point	105-107ºC
Flash Point	211.1±31.5 °C
Vapour Pressure	0.0±2.3 mmHg at 25°C
Index of Refraction	1.554
LogP	-1.03
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	12
Heavy Atom Count	18
Complexity	215
Defined Atom Stereocenter Count	0
SMILES	C(CCN(CCCN)N(N=O)O)CNCCCN
InChi Key	STVYBUMQUAJDER-PEZBUJJGSA-N
InChi Code	InChI=1S/C10H26N6O2/c11-5-3-8-13-7-1-2-9-15(10-4-6-12)16(18)14-17/h13,17H,1-12H2/b16-14-
Chemical Name	(Z)-[3-aminopropyl-[4-(3-aminopropylamino)butyl]amino]-hydroxyimino-oxidoazanium
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	At 37°C and 22–25°C, respectively, spermine NONOate has a half-life of 39 minutes and 230 minutes[1].
References	[1]. Measurement and modeling of nitric oxide release rates for nitric oxide donors. Chem Res Toxicol. 1997 Apr;10(4):408-13. [2]. In vitro comparison of two NONOates (novel nitric oxide donors) on rat pulmonary arteries. Eur J Pharmacol. 1998 Aug 28;356(1):49-57.

Solubility Data

Solubility (In Vitro)	H2O: 100 mg/mL (381.17 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (190.59 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8117 mL	19.0585 mL	38.1170 mL
	5 mM	0.7623 mL	3.8117 mL	7.6234 mL
	10 mM	0.3812 mL	1.9059 mL	3.8117 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.