Sodium copper chlorophyllin B exerts anti-viral effect against influenza virus and HIV, with IC50s values of 50 to 100 μM.

Physicochemical Properties

Molecular Formula	C34H29CUN4NA3O7
Molecular Weight	738.14
CAS #	28302-36-5
Appearance	Black green to black solid powder
Boiling Point	790ºC at 760mmHg
Flash Point	431.6ºC
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Chlorophyll B sodium copper demonstrated influenza virus adsorption at 200 µM (P<0.05). The IC50 and 50% cytotoxic concentration (CC50) of sodium copper chlorophyllin are 50 to 100 µM and 200 to 400 µM, respectively. Sodium chlorophyll B copper also decreased HIV adsorption at 2.5 mM (P<0.05). The IC50 and CC50 of sodium copper chlorophyllin are 50 to 100 µM and 200 to 400 µM respectively[1].
References	[1]. In Vitro Inhibition of Cytopathic Effect of Influenza Virus and Human Immunodeficiency Virus by Bamboo Leaf Extract Solution and Sodium Copper Chlorophyllin. Yonago Acta Med. 2016 Mar; 59(1): 61-65.

Solubility Data

Solubility (In Vitro)

H2O : ~10 mg/mL (~13.55 mM) DMSO : ~6.67 mg/mL (~9.04 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.67 mg/mL (0.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.67 mg/mL (0.91 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 0.67 mg/mL (0.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 20 mg/mL (27.10 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.3548 mL	6.7738 mL	13.5476 mL
	5 mM	0.2710 mL	1.3548 mL	2.7095 mL
	10 mM	0.1355 mL	0.6774 mL	1.3548 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.