Sevasemten is an allosteric inhibitor of skeletal muscle myosin. Sevasemten selectively inhibits myosin with IC50 ≤ 10 μM (bone) or >100 μM (heart).

Physicochemical Properties

Molecular Formula	C16H11F4N5O2
Molecular Weight	381.28445649147
Exact Mass	381.084
CAS #	2417395-15-2
PubChem CID	149205919
Appearance	White to off-white solid powder
LogP	1.7
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	10
Rotatable Bond Count	5
Heavy Atom Count	27
Complexity	590
Defined Atom Stereocenter Count	0
SMILES	C1(=O)N(CC2=CC(F)=CN=C2)N=C(C2=CN=C(OCC(F)(F)F)N=C2)C=C1
InChi Key	XFPJKQRXBAFXNK-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H11F4N5O2/c17-12-3-10(4-21-7-12)8-25-14(26)2-1-13(24-25)11-5-22-15(23-6-11)27-9-16(18,19)20/h1-7H,8-9H2
Chemical Name	2-[(5-fluoropyridin-3-yl)methyl]-6-[2-(2,2,2-trifluoroethoxy)pyrimidin-5-yl]pyridazin-3-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Preparation of substituted pyridazinones for use in the treatment of neuromuscular diseases: World Intellectual Property Organization, WO2021231615. 2021-11-18. [2]. WHO Drug Information-World Health Organization (WHO).

Solubility Data

Solubility (In Vitro)

DMSO : 250 mg/mL (655.69 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.46 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6227 mL	13.1137 mL	26.2274 mL
	5 mM	0.5245 mL	2.6227 mL	5.2455 mL
	10 mM	0.2623 mL	1.3114 mL	2.6227 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.