Sappanone A is a high isoflavanone that displays anti-inflammatory effects by regulating Nrf2 and NF-κB. Sappanone A reduces allergic airway inflammation in ovalbumin-induced asthma.

Physicochemical Properties

Molecular Formula	C16H12O5
Molecular Weight	284.26
Exact Mass	284.068
CAS #	102067-84-5
PubChem CID	9817274
Appearance	Light yellow to yellow solid powder
Density	1.5±0.1 g/cm3
Boiling Point	605.5±55.0 °C at 760 mmHg
Flash Point	233.2±25.0 °C
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.754
LogP	2.49
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	1
Heavy Atom Count	21
Complexity	432
Defined Atom Stereocenter Count	0
SMILES	C1/C(=C\C2=CC(=C(C=C2)O)O)/C(=O)C3=C(O1)C=C(C=C3)O
InChi Key	KVYZXXBTJHJISR-BJMVGYQFSA-N
InChi Code	InChI=1S/C16H12O5/c17-11-2-3-12-15(7-11)21-8-10(16(12)20)5-9-1-4-13(18)14(19)6-9/h1-7,17-19H,8H2/b10-5+
Chemical Name	(3E)-3-[(3,4-dihydroxyphenyl)methylidene]-7-hydroxychromen-4-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Sappanone A exhibits anti-inflammatory effects via modulation of Nrf2 and NF-κB. Int Immunopharmacol. 2015 Sep;28(1):328-36. [2]. Sappanone A Attenuates Allergic Airway Inflammation in Ovalbumin-Induced Asthma. Int Arch Allergy Immunol. 2016;170(3):180-6.
Additional Infomation	Sappanone A has been reported in Caesalpinia pulcherrima, Biancaea sappan, and Biancaea decapetala with data available.

Solubility Data

Solubility (In Vitro)

DMSO :~125 mg/mL (~439.74 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (7.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.32 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.5179 mL	17.5895 mL	35.1791 mL
	5 mM	0.7036 mL	3.5179 mL	7.0358 mL
	10 mM	0.3518 mL	1.7590 mL	3.5179 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.