Salicylic acid-d6 is a deuterated form of Salicylic acid. Salicylic acid inhibits COX-2 activity, and the inhibitory effect is independent of transcription factor (NF-κB) activation.

Physicochemical Properties

Molecular Formula	C7D6O3
Molecular Weight	144.16
Exact Mass	138.031
CAS #	285979-87-5
Related CAS #	3-Indoleacetic acid;87-51-4;Salicylic acid;69-72-7
Appearance	White to off-white solid powder
Density	1.4±0.1 g/cm3
Boiling Point	336.3±0.0 °C at 760 mmHg
Flash Point	144.5±19.1 °C
Vapour Pressure	0.0±0.7 mmHg at 25°C
Index of Refraction	1.616
LogP	2.06
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	COX-2 Autophagy Mitophagy Apoptosis
References	[1]. Sodium salicylate inhibits cyclo-oxygenase-2 activity independently of transcription factor (nuclear factor kappaB) activation: role of arachidonic acid. Mol Pharmacol. 1997 Jun;51(6):907-12.

Solubility Data

Solubility (In Vitro)

DMSO : 250 mg/mL (1734.18 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (14.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (14.43 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (14.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.9367 mL	34.6837 mL	69.3674 mL
	5 mM	1.3873 mL	6.9367 mL	13.8735 mL
	10 mM	0.6937 mL	3.4684 mL	6.9367 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.