Saccharin 1-methylimidazole is an activator of DNA/RNA Synthesis.

Physicochemical Properties

Molecular Formula	C11H11N3O3S
Molecular Weight	265.29
Exact Mass	265.052
CAS #	482333-74-4
PubChem CID	9813926
Appearance	Light yellow to orange solid powder
LogP	0.887
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	0
Heavy Atom Count	18
Complexity	348
Defined Atom Stereocenter Count	0
SMILES	O=S1(NC(=O)C2C=CC=CC1=2)=O.CN1C=NC=C1
InChi Key	DMSNKRPEUQVMNF-UHFFFAOYSA-N
InChi Code	InChI=1S/C7H5NO3S.C4H6N2/c9-7-5-3-1-2-4-6(5)12(10,11)8-7;1-6-3-2-5-4-6/h1-4H,(H,8,9);2-4H,1H3
Chemical Name	1,1-dioxo-1,2-benzothiazol-3-one;1-methylimidazole
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Calcium-deficient T51B cells are stimulated to initiate DNA synthesis rapidly, efficiently, or almost efficiently upon exposure to concentrations of the disparate promoters phenobarbital and the calcium and sodium salts of saccharin (22, 23, 25, 29, 38). This results in an increase in the extracellular calcium concentration to 1.25 mM[1].
References	[1]. Boynton AL, et al. Stimulation of DNA synthesis in calcium-deprived T51B liver cells by the tumor promoters phenobarbital, saccharin, and 12-O-tetradecanoylphorbol-13-acetate. Cancer Res. 1980 Dec;40(12):4541-5.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~376.95 mM) H2O : ≥ 100 mg/mL (~376.95 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.42 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7695 mL	18.8473 mL	37.6946 mL
	5 mM	0.7539 mL	3.7695 mL	7.5389 mL
	10 mM	0.3769 mL	1.8847 mL	3.7695 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.