SN-001 is a STING inhibitor (antagonist) with IC50 of 3.82 μM.

Physicochemical Properties

Molecular Formula	C26H21FN2O4S
Molecular Weight	476.5193
Exact Mass	476.121
Elemental Analysis	C, 65.53; H, 4.44; F, 3.99; N, 5.88; O, 13.43; S, 6.73
CAS #	727699-84-5
PubChem CID	5020799
Appearance	White to off-white solid powder
LogP	4.8
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	7
Heavy Atom Count	34
Complexity	751
Defined Atom Stereocenter Count	0
SMILES	S(C1C([H])=C([H])C(=C([H])C=1[H])F)(N([H])C1=C(C([H])=C([H])C(=C1[H])N([H])C(C1C([H])=C([H])C(=C([H])C=1[H])C1C([H])=C([H])C([H])=C([H])C=1[H])=O)OC([H])([H])[H])(=O)=O
InChi Key	DVLMVJILWFSRPS-UHFFFAOYSA-N
InChi Code	InChI=1S/C26H21FN2O4S/c1-33-25-16-13-22(17-24(25)29-34(31,32)23-14-11-21(27)12-15-23)28-26(30)20-9-7-19(8-10-20)18-5-3-2-4-6-18/h2-17,29H,1H3,(H,28,30)
Chemical Name	N-[3-[(4-fluorophenyl)sulfonylamino]-4-methoxyphenyl]-4-phenylbenzamide
Synonyms	DUN99845; DUN-99845; DUN 99845;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Targeting the human STING's cyclic dinucleotide binding pocket is SN-001 [1]. In a dose-dependent manner, SN-001 (5–20 μM; 6 hours) significantly reduces the induction of Ifnb mRNA in L929 cells [1]. Cytosolic DNA-induced STING signaling is inhibited by SN-001 (10 μM; 3 hours) [1].
Cell Assay	Western Blot Analysis[1] Cell Types: L929 Cell Tested Concentrations: 10 μM Incubation Duration: 3 hrs (hours) Experimental Results: Cytoplasmic DNA-induced reduction in STING, TBK1, IRF3, IκBα and p65 phosphorylation and nuclear translocation of IRF3 and p65.
References	[1]. STING inhibitors target the cyclic dinucleotide binding pocket. Proc Natl Acad Sci U S A. 2021 Jun 15;118(24):e2105465118.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~524.64 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0985 mL	10.4927 mL	20.9855 mL
	5 mM	0.4197 mL	2.0985 mL	4.1971 mL
	10 mM	0.2099 mL	1.0493 mL	2.0985 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.