SR-31747 free base is a sigma ligand with immunosuppressive and anti~inflammatory properties. SR-31747 free base prevents cell growth/proliferation by inhibiting sterol isomerase.

Physicochemical Properties

Molecular Formula	C23H34CLN
Molecular Weight	359.9758
Exact Mass	359.238
CAS #	132173-06-9
Related CAS #	SR-31747;132173-07-0
PubChem CID	6913112
Appearance	Light yellow to yellow liquid
LogP	7.055
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	6
Heavy Atom Count	25
Complexity	395
Defined Atom Stereocenter Count	0
SMILES	ClC1C([H])=C(/C(/[H])=C(/[H])\C([H])([H])N(C([H])([H])C([H])([H])[H])C2([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C2([H])[H])C([H])=C([H])C=1C1([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C1([H])[H]
InChi Key	MYKJVLTXPNIGOV-KTKRTIGZSA-N
InChi Code	InChI=1S/C23H34ClN/c1-2-25(21-13-7-4-8-14-21)17-9-10-19-15-16-22(23(24)18-19)20-11-5-3-6-12-20/h9-10,15-16,18,20-21H,2-8,11-14,17H2,1H3/b10-9-
Chemical Name	N-[(Z)-3-(3-chloro-4-cyclohexylphenyl)prop-2-enyl]-N-ethylcyclohexanamine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	SR-31747 blocks lymphocyte proliferation at a dose of 10 nM. SR-31747 suppresses T cell growth when added 24 hours after activation. SR-31747 suppresses yeast cell growth in a dose-dependent manner [2].
ln Vivo	SR-31747 exhibited a dose-dependent inhibition of lipopolysaccharide-induced IL-1, IL-6, and TNF-α production in vivo (ED50 = 2 mg/kg). Monokine synthesis is inhibited by SR-31747, potentially by a secondary mechanism involving endogenous corticosteroids. In vivo tests that demonstrate that: 1) SR-31747 treatment causes raised corticosterone levels; and 2) corticosteroid removal via mifepristone or adrenalectomy reduces the effects of SR-31747 support this result. By single-factor inhibition, SR-31747 increases the survival of mice that have been exposed to endotoxins [1].
References	[1]. In vivo inhibition of endotoxin-induced pro-inflammatory cytokines production by the sigma ligand SR 31747. J Pharmacol Exp Ther. 1995 Jan;272(1):224-30. [2]. The immunosuppressant SR 31747 blocks cell proliferation by inhibiting a steroid isomerase in Saccharomyces cerevisiae. Mol Cell Biol. 1996 Jun;16(6):2719-27.

Solubility Data

Solubility (In Vitro)

DMSO : ~5 mg/mL (~13.89 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.5 mg/mL (1.39 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.5 mg/mL (1.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 0.5 mg/mL (1.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7779 mL	13.8897 mL	27.7793 mL
	5 mM	0.5556 mL	2.7779 mL	5.5559 mL
	10 mM	0.2778 mL	1.3890 mL	2.7779 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.