Physicochemical Properties
| Molecular Formula | C28H34CL2N6O3 |
| Related CAS # | SPOP-IN-6b;2136270-20-5;SPOP-IN-6b hydrochloride;2136270-68-1 |
| Appearance | Light yellow to yellow solid powder |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | For A498, Caki-2, Ketr-3, 769-P, 0S-RC-2, and 786-0 cells, SPOP-IN-6b dihydrochloride exhibits IC50s of 2-10.2 μM[1]. By suppressing SPOP activity, SPOP-IN-6b(0.1 -3.0 μM; 10 hours) dihydrochloride can raise the amount of DUSP7 and PTEN, two cancer substrate proteins that are suppressed[1]. |
| ln Vivo | SPOP-IN-6b (40–80 mg/kg; daily injection into the peritoneum; 25 days; naked mice) By inhibiting SPOP, dihydrochloride can decrease the growth of tumors[1]. |
| References |
[1]. The pyrido-pyrimidines of 2- imines -5- ketone group -2,5- dihydros -1-H- two. CN107141287A. |
Solubility Data
| Solubility (In Vitro) | DMSO :~41.67 mg/mL (~72.66 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |