SPL-410 is an orally bioactive, potent, selective, hydroxyethylamine-like signal peptide peptidase 2a (SPPL2a) inhibitor (antagonist) with IC50 of 9 nM.

Physicochemical Properties

Molecular Formula	C24H31F3N2O4S
Molecular Weight	500.574156045914
Exact Mass	500.195
CAS #	2351886-00-3
Related CAS #	2351886-00-3;
PubChem CID	138911346
Appearance	White to off-white solid powder
LogP	4.8
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	9
Rotatable Bond Count	9
Heavy Atom Count	34
Complexity	746
Defined Atom Stereocenter Count	2
SMILES	CC(C)(C)C1=CC=C(C=C1)S(=O)(=O)N(C[C@@H]([C@H](C2=CC=C(C=C2)OC(F)(F)F)N)O)C3CCC3
InChi Key	KEEHEUGOPCFXAV-VXKWHMMOSA-N
InChi Code	InChI=1S/C24H31F3N2O4S/c1-23(2,3)17-9-13-20(14-10-17)34(31,32)29(18-5-4-6-18)15-21(30)22(28)16-7-11-19(12-8-16)33-24(25,26)27/h7-14,18,21-22,30H,4-6,15,28H2,1-3H3/t21-,22-/m0/s1
Chemical Name	N-[(2S,3S)-3-amino-2-hydroxy-3-[4-(trifluoromethoxy)phenyl]propyl]-4-tert-butyl-N-cyclobutylbenzenesulfonamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Solubility Data

Solubility (In Vitro)

DMSO : ~190 mg/mL (~379.57 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 4.75 mg/mL (9.49 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 47.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9977 mL	9.9886 mL	19.9772 mL
	5 mM	0.3995 mL	1.9977 mL	3.9954 mL
	10 mM	0.1998 mL	0.9989 mL	1.9977 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.