SM-433 hydrochlorid is a Smac mimetic that works as an inhibitor (blocker/antagonist) of inhibitory apoptosis proteins (IAPs). SM-433 hydrochlorid displays strong binding affinity to XIAP BIR3 protein with IC50 <1 μM (details from patent WO2008128171A2).

Physicochemical Properties

Molecular Formula	C32H44CLN5O4
Molecular Weight	598.18
Appearance	Off-white to light brown solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	SM-433 hydrochlorid demonstrates potent inhibitory action on SK-OV-3 ovarian cancer cells and MDA-MB -2131 human breast cancer cells (IC50s<10 μM, respectively)[1].
References	[1]. Diazo bicyclic smac mimetics and the uses thereof. WO2008128171A2.

Solubility Data

Solubility (In Vitro)

DMSO :~180 mg/mL (~300.91 mM) H2O :~25 mg/mL (~41.79 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 4.5 mg/mL (7.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 45.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 4.5 mg/mL (7.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 45.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 4.5 mg/mL (7.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 45.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.6717 mL	8.3587 mL	16.7174 mL
	5 mM	0.3343 mL	1.6717 mL	3.3435 mL
	10 mM	0.1672 mL	0.8359 mL	1.6717 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.