SLK/STK10-IN-1 is a potent and specific SLK and STK10 inhibitor (antagonist) with nanomolar potency.

Physicochemical Properties

Molecular Formula	C17H13N2O3CL
Molecular Weight	328.75
Exact Mass	328.061
CAS #	264210-49-3
PubChem CID	6539459
Appearance	White to yellow solid powder
LogP	3.3
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	4
Heavy Atom Count	23
Complexity	520
Defined Atom Stereocenter Count	0
SMILES	ClC1C=CC=C(NC2=C(C3=CC=CC=C3OC)C(=O)NC2=O)C=1
InChi Key	DQYDZCRLDYVZKY-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H13ClN2O3/c1-23-13-8-3-2-7-12(13)14-15(17(22)20-16(14)21)19-11-6-4-5-10(18)9-11/h2-9H,1H3,(H2,19,20,21,22)
Chemical Name	3-(3-chloroanilino)-4-(2-methoxyphenyl)pyrrole-2,5-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Discovery of a Potent Dual SLK/STK10 Inhibitor Based on a Maleimide Scaffold. J Med Chem. 2021 Sep 23;64(18):13259-13278.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (304.18 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.60 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0418 mL	15.2091 mL	30.4183 mL
	5 mM	0.6084 mL	3.0418 mL	6.0837 mL
	10 mM	0.3042 mL	1.5209 mL	3.0418 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.