SJ10542 is a potent and specific JAK2/3-directed phenylglutarimide (PG)-PROTAC with DC50s of 14, 11, and 24 nM for JAK2, JAK3, and JAK2-fusion ALL, respectively. SJ10542 utilizes PG ligands as cereblon (CRBN) recruiters.

Physicochemical Properties

Molecular Formula	C41H46N12O5S
Molecular Weight	818.946345806122
Exact Mass	818.343
CAS #	2789678-92-6
PubChem CID	162679331
Appearance	White to off-white solid powder
LogP	1.8
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	13
Rotatable Bond Count	14
Heavy Atom Count	59
Complexity	1640
Defined Atom Stereocenter Count	0
InChi Key	WBEBZVDAHMXWJN-UHFFFAOYSA-N
InChi Code	InChI=1S/C41H46N12O5S/c1-2-59(57,58)52-26-41(27-52,15-16-42)53-25-30(24-45-53)36-34-14-18-43-37(34)49-40(48-36)46-31-8-4-29(5-9-31)38(55)44-17-3-19-50-20-22-51(23-21-50)32-10-6-28(7-11-32)33-12-13-35(54)47-39(33)56/h4-11,14,18,24-25,33H,2-3,12-13,15,17,19-23,26-27H2,1H3,(H,44,55)(H,47,54,56)(H2,43,46,48,49)
Chemical Name	4-[[4-[1-[3-(cyanomethyl)-1-ethylsulfonylazetidin-3-yl]pyrazol-4-yl]-7H-pyrrolo[2,3-d]pyrimidin-2-yl]amino]-N-[3-[4-[4-(2,6-dioxopiperidin-3-yl)phenyl]piperazin-1-yl]propyl]benzamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	JAK2 14 nM (DC50) JAK3 11 nM (DC50) JAK2-fusion ALL 24 nM (DC50) Cereblon
References	[1]. Development of Potent and Selective Janus Kinase 2/3 Directing PG-PROTACs.ACS Med.Chem.Lett.2022,13,3,475-482.

Solubility Data

Solubility (In Vitro)

DMSO : 46.67 mg/mL (56.99 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (3.05 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.2211 mL	6.1054 mL	12.2108 mL
	5 mM	0.2442 mL	1.2211 mL	2.4422 mL
	10 mM	0.1221 mL	0.6105 mL	1.2211 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.