SB297006 (SB-297006) is an antagonist of CCR3 (Chemokine receptor, IC50 = 39 nM) with anticancer activity. It dramatically suppresses neurosphere formation and proliferation in neural progenitor cells treated with CCL11. Leukocytes have CCR3, which is the main receptor for CCL11. Neural progenitor cells (NPCs) exhibited the functional chemokine receptor CCR3, and when CCL11 was applied to NPCs, the CCR3 receptor antagonist SB297006 dramatically reduced NPC proliferation and neurosphere formation.

Physicochemical Properties

Molecular Formula	C₁₈H₁₈N₂O₅
Molecular Weight	342.35
Exact Mass	342.122
Elemental Analysis	C, 63.15; H, 5.30; N, 8.18; O, 23.37
CAS #	58816-69-6
Related CAS #	58816-69-6
PubChem CID	9840971
Appearance	White to off-white solid powder
Melting Point	121 °C
LogP	3.413
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	7
Heavy Atom Count	25
Complexity	463
Defined Atom Stereocenter Count	1
SMILES	O(C([H])([H])C([H])([H])[H])C([C@]([H])(C([H])([H])C1C([H])=C([H])C(=C([H])C=1[H])[N+](=O)[O-])N([H])C(C1C([H])=C([H])C([H])=C([H])C=1[H])=O)=O
InChi Key	BEZXGSZPWXRHIN-INIZCTEOSA-N
InChi Code	InChI=1S/C18H18N2O5/c1-2-25-18(22)16(19-17(21)14-6-4-3-5-7-14)12-13-8-10-15(11-9-13)20(23)24/h3-11,16H,2,12H2,1H3,(H,19,21)/t16-/m0/s1
Chemical Name	ethyl (2S)-2-benzamido-3-(4-nitrophenyl)propanoate
Synonyms	SB-297006; SB 297006; SB297006
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	CCR3
ln Vitro	SB297006 is a CCR3 antagonist dramatically reduces neurosphere formation and proliferation in CCL11-treated neural progenitor cells (NPCs) at 100 μM[1].
Cell Assay	Neural progenitor cells (NPCs) are stimulated with CCL11 (2 μg/mL) after 30 minutes of incubation with the CCR3 antagonist SB297006 (100 μM) to assess CCR3 blocking. NPCs are subjected to CCK-8 assays following a 3-day culture period.
References	[1]. CCL11 promotes migration and proliferation of mouse neural progenitor cells. Stem Cell Res Ther. 2017 Feb 7;8(1):26.

Solubility Data

Solubility (In Vitro)

DMSO: 68~150 mg/mL (198.6~438.2 mM） Water: <1 mg/mL Ethanol: <1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9210 mL	14.6049 mL	29.2099 mL
	5 mM	0.5842 mL	2.9210 mL	5.8420 mL
	10 mM	0.2921 mL	1.4605 mL	2.9210 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.