SATA Protein Modifier, or N-Succinimidyl S-Acetylthioacetate, is a protein modification reagent used to convert primary amine groups into protected sulfhydryls (thiol; -SH) for later reaction with sulfhydryl reactive crosslinkers such as Sulfo-SMCC, Sulfo-MBS, etc.
Physicochemical Properties
| Molecular Formula | C8H9NO5S |
| Molecular Weight | 231.2258 |
| Exact Mass | 231.02 |
| CAS # | 76931-93-6 |
| Related CAS # | 76931-93-6; |
| PubChem CID | 127532 |
| Appearance | White to yellow solid powder |
| Density | 1.5±0.1 g/cm3 |
| Boiling Point | 337.3±44.0 °C at 760 mmHg |
| Melting Point | 84-89ºC |
| Flash Point | 157.8±28.4 °C |
| Vapour Pressure | 0.0±0.7 mmHg at 25°C |
| Index of Refraction | 1.562 |
| LogP | -1.16 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 5 |
| Heavy Atom Count | 15 |
| Complexity | 311 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | FLCQLSRLQIPNLM-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C8H9NO5S/c1-5(10)15-4-8(13)14-9-6(11)2-3-7(9)12/h2-4H2,1H3 |
| Chemical Name | (2,5-dioxopyrrolidin-1-yl) 2-acetylsulfanylacetate |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.(2). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| References |
[1]. Interference of ϵ-amino-caproic acid with [N-succinimidyl S-acetylthioacetate derived] thiol-introduction into glu-plasminogen. Volume 8, Supplement 2, 1994, Pages 138-141. [2]. Evaluation of N-Succinimidyl S-Acetylthioacetate Ligand for Radiolabeling of HumanizedAntibodies with 188Rhenium. Cancer Biother Radiopharm. 2018 Oct;33(8):349-355. |
Solubility Data
| Solubility (In Vitro) | DMSO : ~100 mg/mL (~432.47 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.81 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.81 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (10.81 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3247 mL | 21.6235 mL | 43.2470 mL | |
| 5 mM | 0.8649 mL | 4.3247 mL | 8.6494 mL | |
| 10 mM | 0.4325 mL | 2.1623 mL | 4.3247 mL |