SARS-CoV-IN-2 is a potent inhibitor of SARS-CoV replication. In Vero cells, SARS-CoV-IN 1 is antiviral with EC50 of 1.9 μM. SARS-CoV-IN-2 inhibits P. falciparum 3D7 and W2 strains with IC50s of 21.5 and 30 nM, respectively; IC90s of 51.0 and 99.9 nM, respectively. In MT-4 cells, SARS-CoV-IN-2 reduced HIV-1-induced cytopathic effects with EC50 of 2.9 μM. Has antimalarial and anti-viral effect.

Physicochemical Properties

Molecular Formula	C24H18CLFEN3O
Molecular Weight	455.72
CAS #	888958-26-7
Appearance	Light yellow to yellow solid powder
SMILES	C(N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)O)CC(C)C)(=O)[C@]([H])([C@H](O)C)NC(=O)[C@H](C)NC([C@@H]1CCCN1C(=O)CNC(=O)CNC(=O)CN)=O
Synonyms	SARSCoVIN2; SARS CoV IN 2
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	SARS-CoV-IN-2 (compound 3) is significantly more effective than chloroquine (CQ) at preventing Plasmodium falciparum from growing in vitro [1].
References	[1]. Design and synthesis of hydroxyferroquine derivatives with antimalarial and antiviral activities. J Med Chem. 2006 May 4;49(9):2845-9.

Solubility Data

Solubility (In Vitro)

DMSO : ~19.23 mg/mL (~42.20 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.92 mg/mL (4.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 19.2 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1943 mL	10.9716 mL	21.9433 mL
	5 mM	0.4389 mL	2.1943 mL	4.3887 mL
	10 mM	0.2194 mL	1.0972 mL	2.1943 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.