SARS-CoV-IN-1 is a potent inhibitor of SARS-CoV replication. In Vero cells, SARS-CoV-IN-1 is antiviral with EC50 of 4.9 μM. SARS-CoV-IN-1 inhibits P. falciparum 3D7 and W2 strains with IC50s of 15.4 and 133.2 nM respectively; IC90s of 25.7 and 459.1 nM respectively. Has antimalarial and anti-viral effect.

Physicochemical Properties

Molecular Formula	C23H24CLFEN3O
Molecular Weight	441.70
CAS #	888958-25-6
Appearance	Light yellow to yellow solid powder
Synonyms	SARSCoVIN1; SARS CoV IN 1
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In vitro, SARS-CoV-IN-1 (compound 2) inhibits Plasmodium falciparum development significantly more effectively than chloroquine (CQ) [1].
References	[1]. Design and synthesis of hydroxyferroquine derivatives with antimalarial and antiviral activities. J Med Chem. 2006 May 4;49(9):2845-9.

Solubility Data

Solubility (In Vitro)

DMSO : ~41.67 mg/mL (~94.34 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 4.17 mg/mL (9.44 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 41.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 4.17 mg/mL (9.44 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 41.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2640 mL	11.3199 mL	22.6398 mL
	5 mM	0.4528 mL	2.2640 mL	4.5280 mL
	10 mM	0.2264 mL	1.1320 mL	2.2640 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.