SARS-CoV-2 PLpro-IN-1 (Compound 85) is a non-covalent competitive inhibitor of SARS-CoV-2 PLpro with IC50 of 15.06 μM and Ki of 22.93 μM. SARS-CoV-2 PLpro-IN-1 inhibits Vero cell proliferation with IC50 of 7.47 μM.

Physicochemical Properties

Molecular Formula	C18H19N5O
Molecular Weight	321.38
CAS #	3053753-61-7
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 15.06 μM (SARS-CoV-2 PLpro)
References	[1]. Discovery of Non-Covalent Inhibitors for SARS-CoV-2 PLpro: Integrating Virtual Screening, Synthesis, and Experimental Validation. ACS Med Chem Lett. 2024 Dec 2;15(12):2140-2149.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1116 mL	15.5579 mL	31.1158 mL
	5 mM	0.6223 mL	3.1116 mL	6.2232 mL
	10 mM	0.3112 mL	1.5558 mL	3.1116 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.