Physicochemical Properties
| Molecular Formula | C34H43FN4O7 |
| Molecular Weight | 638.73 |
| Appearance | Typically exists as solid at room temperature |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | MPro/CatL[1] |
| ln Vitro | In A549-hACE2 cells, SARS-CoV-2 Mpro-IN-5(SM142) inhibits SARS-CoV2 replication with an IC50 value of 14.7 nM[1]. A549-hACE2 cells are cytotoxically exposed to SARS-CoV-2 Mpro-IN-5(0-50 μM, 24 h)[1]. A549 cells' OC-43 virus mRNA expression is inhibited by SARS-CoV-2 Mpro-IN-5[1]. MPro and CatL are both inhibited by SARS-CoV-2 Mpro-IN-5, which prevents SARS-CoV2 infection[1]. |
| ln Vivo | SARS-CoV-2 Mpro-IN-5 (SM142) protects K18-ACE2 mice against mortality and weight loss caused by SARS-CoV-2 (10 mg/kg for in or 25 mg/kg for ip)[1]. In male C57Bl/6 mice, SARS-CoV -2 Mpro-IN-5 (3 mg/kg, iv) has a half-life of 2.1 h and a high clearance of 18490 mL/min/kg[1]. Oral bioavailability of SARS-CoV-2 Mpro-IN-5 (10 mg/kg, po) is 37.5%[1]. |
| Cell Assay |
Cell Viability Assay[1] Cell Types: A549-hACE2 cells Tested Concentrations: 0, 0.1, 0.2, 2, 20, 50 μM Incubation Duration: 24 h Experimental Results: Dose-dependently inhibited cell viability, reduce by 15% at 50 μM. |
| Animal Protocol |
Animal/Disease Models: K18-hACE2 transgenic mice[1] Doses: 10 mg/kg (in) or 25 mg/kg (ip) Route of Administration: Intranasal inhalation (in), one time/day for 3 days, prior to the infection; or intraperitoneal (ip)injection, twice (two times) daily for 5 days, postinfection administration. Experimental Results: Prevented weight loss and prolonged survival. |
| References | [1]. Mondal S, et al. Dual Inhibitors of Main Protease (MPro) and Cathepsin L as Potent Antivirals against SARS-CoV2. J Am Chem Soc. 2022 Nov 23;144(46):21035-21045. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5656 mL | 7.8280 mL | 15.6561 mL | |
| 5 mM | 0.3131 mL | 1.5656 mL | 3.1312 mL | |
| 10 mM | 0.1566 mL | 0.7828 mL | 1.5656 mL |