S19-1035 is a potent and specific inhibitor of aldehyde-keto reductase 1C3 (AKR1C3). S19-1035 inhibits AKR1C3 with IC50 of 3.04 nM. S19-1035 may be utilized in tumor research.

Physicochemical Properties

Molecular Formula	C19H17CLN2O3
Molecular Weight	356.8
CAS #	2986319-18-8
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 3.04 nM (AKR1C3)[1]
ln Vitro	S19-1035 exhibits 3.04 nM IC50 inhibitory action against AKR1C3[1]. When taken alone, S19-1035 (0-100 μM; 72 h or 96 h) has a limited anti-tumor impact and low cytotoxicity [1]. S19-1035 (10 μM; 8 days) dramatically reverses drug-resistant breast cancer cell lines' resistance to doxorubicin (DOX) [1].
Cell Assay	Cell Cytotoxicity Assay[1] Cell Types: MDA-MB-231, MCF-7 and MCF-7/DOX cells Tested Concentrations: 0-100 μM Incubation Duration: 72 h or 96 h Experimental Results: Had weak antiproliferative effects in all three breast cancer cell lines.
References	[1]. Development of highly potent and specific AKR1C3 inhibitors to restore the chemosensitivity of drug-resistant breast cancer. Eur J Med Chem. 2022 Dec 13;247:115013.

Solubility Data

Solubility (In Vitro)

DMSO :~125 mg/mL (~350.34 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.08 mg/mL (5.83 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.83 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8027 mL	14.0135 mL	28.0269 mL
	5 mM	0.5605 mL	2.8027 mL	5.6054 mL
	10 mM	0.2803 mL	1.4013 mL	2.8027 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.