Miltirone is a naturally occurring compound found in salvia. Miltirone works as a coagulant at the central azepine receptor (benzodiazepine receptor) portion, with IC50 of 0.3 μM.

Physicochemical Properties

Molecular Formula	C19H22O2
Molecular Weight	282.3768
Exact Mass	282.161
CAS #	27210-57-7
PubChem CID	160142
Appearance	Orange to red solid powder
Density	1.1±0.1 g/cm3
Boiling Point	421.6±45.0 °C at 760 mmHg
Melting Point	98-100℃ (hexane )
Flash Point	179.4±14.4 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.556
LogP	5.67
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	1
Heavy Atom Count	21
Complexity	498
Defined Atom Stereocenter Count	0
InChi Key	FEFAIBOZOKSLJR-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H22O2/c1-11(2)14-10-12-7-8-15-13(6-5-9-19(15,3)4)16(12)18(21)17(14)20/h7-8,10-11H,5-6,9H2,1-4H3
Chemical Name	8,8-dimethyl-2-propan-2-yl-6,7-dihydro-5H-phenanthrene-3,4-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Miltirone, a central benzodiazepine receptor partial agonist from a Chinese medicinal herb Salvia miltiorrhiza. Neurosci Lett. 1991 Jun 24;127(2):237-41.
Additional Infomation	Miltirone is an abietane diterpenoid. Miltirone has been reported in Salvia miltiorrhiza, Salvia officinalis, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~177.07 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (8.85 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (8.85 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.5413 mL	17.7066 mL	35.4133 mL
	5 mM	0.7083 mL	3.5413 mL	7.0827 mL
	10 mM	0.3541 mL	1.7707 mL	3.5413 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.