Rosarin is a naturally occurring cinnamyl alcohol glycoside found in Rhodiola rosea. It exhibits anti-inflammatory and neuroprotective activity by suppressing the expression of the proinflammatory factors such as iNOS, IL-1 β, and TNF-α.

Physicochemical Properties

Molecular Formula	C20H28O10
Molecular Weight	428.4303
Exact Mass	428.168
CAS #	84954-93-8
PubChem CID	10320370
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Boiling Point	730.8±60.0 °C at 760 mmHg
Flash Point	395.8±32.9 °C
Vapour Pressure	0.0±2.5 mmHg at 25°C
Index of Refraction	1.635
LogP	2.03
Hydrogen Bond Donor Count	6
Hydrogen Bond Acceptor Count	10
Rotatable Bond Count	8
Heavy Atom Count	30
Complexity	540
Defined Atom Stereocenter Count	9
SMILES	C1=CC=C(C=C1)/C=C/CO[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CO[C@H]3[C@@H]([C@H]([C@@H](O3)CO)O)O)O)O)O
InChi Key	IEBFEMIXXHIISM-YZOUKVLTSA-N
InChi Code	InChI=1S/C20H28O10/c21-9-12-14(22)17(25)20(29-12)28-10-13-15(23)16(24)18(26)19(30-13)27-8-4-7-11-5-2-1-3-6-11/h1-7,12-26H,8-10H2/b7-4+/t12-,13+,14-,15+,16-,17+,18+,19+,20+/m0/s1
Chemical Name	(2R,3S,4S,5R,6R)-2-[[(2R,3R,4R,5S)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxymethyl]-6-[(E)-3-phenylprop-2-enoxy]oxane-3,4,5-triol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The production of iNOS and cytokines in mouse BV2 cells was reduced in a concentration-dependent manner by rosarine (0-50 μg/ml; 18 hours) pretreatment for 30 minutes [1].
References	[1]. Anti-Inflammatory and Neuroprotective Effects of Constituents Isolated from Rhodiola rosea.Evid Based Complement Alternat Med. 2013;2013:514049.
Additional Infomation	Rosarin is an O-acyl carbohydrate. Rosarin has been reported in Rhodiola crenulata, Rhodiola sachalinensis, and other organisms with data available. See also: Sedum roseum root (part of).

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~233.41 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.84 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (5.84 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3341 mL	11.6705 mL	23.3410 mL
	5 mM	0.4668 mL	2.3341 mL	4.6682 mL
	10 mM	0.2334 mL	1.1671 mL	2.3341 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.