Rhodojaponin V is a diterpenoid obtained from the leaves of Rhododendron molle and has anti~inflammatory activity.

Physicochemical Properties

Molecular Formula	C22H34O7
Molecular Weight	410.5012
Exact Mass	410.23
CAS #	37720-86-8
PubChem CID	169961
Appearance	White to off-white solid powder
Density	1.4±0.1 g/cm3
Boiling Point	551.8±50.0 °C at 760 mmHg
Melting Point	232 °C
Flash Point	187.5±23.6 °C
Vapour Pressure	0.0±3.4 mmHg at 25°C
Index of Refraction	1.603
LogP	1.51
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	2
Heavy Atom Count	29
Complexity	768
Defined Atom Stereocenter Count	0
InChi Key	OHDPFRGZBUACTR-UHFFFAOYSA-N
InChi Code	InChI=1S/C22H34O7/c1-10(23)28-16-11-6-7-12-20(5,26)15-14-17(29-14)18(2,3)22(15,27)13(24)8-21(12,16)9-19(11,4)25/h11-17,24-27H,6-9H2,1-5H3
Chemical Name	(3,4,10,15-tetrahydroxy-5,5,10,15-tetramethyl-7-oxapentacyclo[12.2.1.01,11.04,9.06,8]heptadecan-17-yl) acetate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Anti-inflammatory Grayanane Diterpenoids from the Leaves of Rhododendron molle. J Nat Prod. 2018 Jan 26;81(1):151-161.
Additional Infomation	5,6,10,16-Tetrahydroxy-2,3-epoxygrayanotoxan-14-yl acetate has been reported in Rhododendron japonicum with data available. See also: Rhodojaponin V (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~121.80 mM) H2O : ~1 mg/mL (~2.44 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (6.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4361 mL	12.1803 mL	24.3605 mL
	5 mM	0.4872 mL	2.4361 mL	4.8721 mL
	10 mM	0.2436 mL	1.2180 mL	2.4361 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.