Resorufin sodium salt (NSC 12097 sodium salt) is a highly fluorescent pink dye for the detection of ROS/RNS and second analytes.

Physicochemical Properties

Molecular Formula	C12H6NO3-.NA+
Molecular Weight	235.17074
Exact Mass	235.025
CAS #	34994-50-8
Related CAS #	Resorufin;635-78-9
PubChem CID	23693100
Appearance	Dark purple to black solid powder
Melting Point	>300ºC(lit.)
LogP	2.436
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	0
Heavy Atom Count	17
Complexity	428
Defined Atom Stereocenter Count	0
InChi Key	TYXBZZBYMSVFPR-UHFFFAOYSA-M
InChi Code	InChI=1S/C12H7NO3.Na/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9;/h1-6,14H;/q;+1/p-1
Chemical Name	sodium;7-oxophenoxazin-3-olate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. 'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte. Chem Commun (Camb). 2018 Jul 26;54(61):8466-8469.

Solubility Data

Solubility (In Vitro)

DMSO : ~62.5 mg/mL (~265.77 mM) H2O : ~7.69 mg/mL (~32.70 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (8.84 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2522 mL	21.2612 mL	42.5224 mL
	5 mM	0.8504 mL	4.2522 mL	8.5045 mL
	10 mM	0.4252 mL	2.1261 mL	4.2522 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.