RdRP-IN-9 (Compound 6b) is a reversible covalent RdRp allosteric inhibitor. RdRP-IN-9 exhibits high anti-coronavirus activity, with an EC50 value of 0.68 μM against HCoV-OC43. RdRP-IN-9 can reversibly acylate Cys12 of the NiRAN domain in nonstructural protein 12 (nsp 12), thereby exerting a more persistent antiviral effect, and exhibits synergistic anti-coronavirus activity with Molnupiravir.

Physicochemical Properties

Molecular Formula	C19H21NO5
Molecular Weight	343.37
CAS #	1101848-34-3
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	EC50: 0.68 μM (HCoV-OC43)[1].
References	[1]. Lycorine esters exert anti-HCoV-OC43 effect through reversibly acylating cysteine residue in the nsp 12 NiRAN domainJ. Bioorganic Chemistry, 2024: 108086.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9123 mL	14.5616 mL	29.1231 mL
	5 mM	0.5825 mL	2.9123 mL	5.8246 mL
	10 mM	0.2912 mL	1.4562 mL	2.9123 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.