RTx-161 is an allosteric Polθ polymerase inhibitor with an IC50 of 4.1 nM. RTx-161 selectively kills HR-deficient cancer cells and suppresses PARP inhibitor (PARPi) resistance in multiple genetic backgrounds including HR-sufficient cells. RTx-161 also induces apoptosis.

Physicochemical Properties

Molecular Formula	C21H18F7N3O4
Molecular Weight	509.37
Appearance	Colorless to off-white solid powder
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Discovery of a small-molecule inhibitor that traps Polθ on DNA and synergizes with PARP inhibitorsJ. Nature communications, 2024, 15(1): 2862.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9632 mL	9.8160 mL	19.6321 mL
	5 mM	0.3926 mL	1.9632 mL	3.9264 mL
	10 mM	0.1963 mL	0.9816 mL	1.9632 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.