RIPK1-IN-28 (compound 13) is an orally active RIPK1 inhibitor. RIPK1-IN-28 inhibits human I2.1 and Hepa1-6 cells with IC50 of 0.4 and 1.2 nM, respectively.

Physicochemical Properties

Molecular Formula	C27H24N4O4
Molecular Weight	468.50
CAS #	2968509-28-4
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Design and synthesis of pyridazin-4-one derivatives as necroptosis inhibitors. Arch Pharm (Weinheim). 2025 Jan;358(1):e2400594.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1345 mL	10.6724 mL	21.3447 mL
	5 mM	0.4269 mL	2.1345 mL	4.2689 mL
	10 mM	0.2134 mL	1.0672 mL	2.1345 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.