RFRP-3 (mouse) is a functional homolog of avian gonadotropin-inhibiting hormone (GnIH) and binds to GPR147. RFRP-3 (mouse) reduces progesterone synthesis by inhibiting FSHR and key enzymes involved in steroidogenesis (P450scc, 3β-HSD, StAR). RFRP-3 (mouse) induces apoptosis (increase in p53). RFRP-3 (mouse) also inhibits the ERK signaling pathway. RFRP-3 can be used to study follicle development.

Physicochemical Properties

Molecular Formula	C91H141N29O25S
Molecular Weight	2073.34
Synonyms	Neuropeptide NPVF (mouse)
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	RFRP-3 (10-1000 ng/mL, 24 h) can significantly reduce the secretion of progesterone, but not estradiol, in mouse ovarian granulosa cells[1]. RFRP-3 (10-1000 ng/mL, 24 h) inhibits steroidogenesis and FSHR in mouse ovarian granulosa cells (inhibits p450scc, 3β-HSD, StAR and FSHR)[1]. RFRP-3 (10-1000 ng/mL, 24 h) induces apoptosis in mouse ovarian granulosa cells and reduces Bcl-2 protein expression and increases p53 protein expression at moderate doses [1]. RFRP-3 (10-1000 ng/mL, 24 h) inhibits the ERK signaling pathway (p-ERK1/2 protein) in mouse ovarian granulosa cells[1].
Cell Assay	Real Time qPCR[1] Cell Types: Granulosa cells Tested Tested Concentrations: 10, 100, 1000 ng/mL Incubation Duration: 24 h Experimental Results: Reduced p450scc mRNA expression at 100 ng/mL, but did not significantly alter p450scc expression at low dose (10 ng/mL) and high dose (1000 ng/mL). Reduced 3β-HSD, FSHR and StAR expression at 100 ng/mL and 1000 ng/mL, whereas had no significant effect at 10 ng/mL.
References	[1]. Rfamide-related peptide-3(RFRP-3) receptor gene is expressed in mouse ovarian granulosa cells: Potential role of RFRP-3 in steroidogenesis and apoptosis. Steroids. 2024 Feb;202:109349.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.4823 mL	2.4116 mL	4.8231 mL
	5 mM	0.0965 mL	0.4823 mL	0.9646 mL
	10 mM	0.0482 mL	0.2412 mL	0.4823 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.