Pyribencarb is a benzyl carbamate-type fungicide active against a variety of phytopathogenic fungi. Pyribencarb is a potent Qo inhibitor of cytochrome b. Pyribencarb is particularly effective against Botrytis cinerea and Sclerotinia sclerotiorum.

Physicochemical Properties

Molecular Formula	C18H20CLN3O3
Molecular Weight	361.83
Exact Mass	361.119
CAS #	799247-52-2
PubChem CID	11577870
Appearance	Off-white to light yellow solid powder
Density	1.221g/cm3
Index of Refraction	1.57
LogP	4.044
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	7
Heavy Atom Count	25
Complexity	461
Defined Atom Stereocenter Count	0
SMILES	C(/C1C=CC(Cl)=C(CNC(=O)OC)C=1)(\C)=N\OCC1C=CC=C(C)N=1
InChi Key	CRFYLQMIDWBKRT-LPYMAVHISA-N
InChi Code	InChI=1S/C18H20ClN3O3/c1-12-5-4-6-16(21-12)11-25-22-13(2)14-7-8-17(19)15(9-14)10-20-18(23)24-3/h4-9H,10-11H2,1-3H3,(H,20,23)/b22-13+
Chemical Name	methyl N-[[2-chloro-5-[(E)-C-methyl-N-[(6-methylpyridin-2-yl)methoxy]carbonimidoyl]phenyl]methyl]carbamate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Pyribencarb is an effective inhibitor of the decylubiquinol-cytochrome c reductase (UCR) activity of B. cinerea and Corynespora cassiicola, as well as the succinate cytochrome c reductase (SCR) activity of B. cinerea and Corynespora cassiicola. cinerea. Pyribencarb prevents B's UCR function. cinerea, with an estimated substrate-dependent inhibition constant of 13 nM, in a non-competitive manner [1].
References	[1]. Satoshi Kataoka, et al. Mechanism of action and selectivity of a novel fungicide, pyribencarb. J Pestic Sci, 35(2), 99-106 (2010).
Additional Infomation	Pyribencarb is a carbamate ester that is the methyl ester of (2-chloro-5-{(1E)-N-[(6-methylpyridin-2-yl)methoxy]ethanimidoyl}benzyl)carbamic acid. A fungicide having excellent activity against a wide range of plant pathogenic fungi, especially gray mould diseases caused by Botrytis cinerea. It has a role as a mitochondrial cytochrome-bc1 complex inhibitor and an antifungal agrochemical. It is a carbamate fungicide and a methyl (2-chloro-5-{N-[(6-methylpyridin-2-yl)methoxy]ethanimidoyl}benzyl)carbamate.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (276.37 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.91 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (6.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7637 mL	13.8186 mL	27.6373 mL
	5 mM	0.5527 mL	2.7637 mL	5.5275 mL
	10 mM	0.2764 mL	1.3819 mL	2.7637 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.