Pseudoisocyanine (iodide) is a pan-inhibitor of monoamine transporters and organic cation transporters with antidepressant activity.

Physicochemical Properties

Molecular Formula	C23H23IN2
Molecular Weight	454.3466
Exact Mass	454.091
CAS #	977-96-8
Related CAS #	20766-49-8 (Parent)
PubChem CID	5484462
Appearance	Brown to dark brown solid powder
Melting Point	273ºC
LogP	1.235
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	3
Heavy Atom Count	26
Complexity	464
Defined Atom Stereocenter Count	0
SMILES	CCN1/C(=C/C2=[N+](C3=CC=CC=C3C=C2)CC)/C=CC4=CC=CC=C41.[I-]
InChi Key	GMYRVMSXMHEDTL-UHFFFAOYSA-M
InChi Code	InChI=1S/C23H23N2.HI/c1-3-24-20(15-13-18-9-5-7-11-22(18)24)17-21-16-14-19-10-6-8-12-23(19)25(21)4-2;/h5-17H,3-4H2,1-2H3;1H/q+1;/p-1
Chemical Name	(2E)-1-ethyl-2-[(1-ethylquinolin-1-ium-2-yl)methylidene]quinoline;iodide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Comparative analysis of novel decynium-22 analogs to inhibit transport by the low-affinity, high-capacity monoamine transporters, organic cation transporters 2 and 3, and plasma membrane monoamine transporter. Eur J Pharmacol. 2019 Jan 5;842:351-364.
Additional Infomation	1,1'-diethyl-2,2'-cyanine iodide is a 1,1'-diethyl-2,2'-cyanine halide and an organic iodide salt.

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~55.02 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.50 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2009 mL	11.0047 mL	22.0095 mL
	5 mM	0.4402 mL	2.2009 mL	4.4019 mL
	10 mM	0.2201 mL	1.1005 mL	2.2009 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.