Povidone iodine (iodopovidone) has excellent anti-bacterial effect and can kill MRSA and MSSA strains with MICs of 31.25 mg/L and 7.82 mg/L respectively.

Physicochemical Properties

Molecular Formula	C6H9I2NO
Molecular Weight	364.9507
Exact Mass	364.877
CAS #	25655-41-8
PubChem CID	410087
Appearance	Typically exists as solid at room temperature
Boiling Point	217.6ºC at 760 mmHg
Melting Point	300ºC
Flash Point	93.9ºC
Vapour Pressure	0.132mmHg at 25°C
LogP	2.461
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	1
Heavy Atom Count	10
Complexity	120
Defined Atom Stereocenter Count	0
SMILES	II.O=C1C([H])([H])C([H])([H])C([H])([H])N1C([H])=C([H])[H]
InChi Key	CPKVUHPKYQGHMW-UHFFFAOYSA-N
InChi Code	InChI=1S/C6H9NO.I2/c1-2-7-5-3-4-6(7)8;1-2/h2H,1,3-5H2;
Chemical Name	1-ethenylpyrrolidin-2-one;molecular iodine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The MIC values of povidone-iodine (PVP-I) are 31.25 mg/L and 7.82 mg/L respectively. Treatment of cells with povidone-iodine (PVP-I) at a 1:32 dilution resulted in a substantial 90-95% drop in cell viability in all cell lines examined [1].
ln Vivo	The group that received Dp + povidone-iodine (PVP-I) had the second-highest mean score from day 13 until the conclusion of the study period. Additionally, compared to the control group, the Dp+povidone-iodine and Dp+Et-OH groups had significantly higher eosinophil counts (p<0.05 and p<0.001, respectively). The eosinophil counts of the Dp+povidone-iodine (PVP-I), Dp+CHG, and Dp+vehicle groups did not, however, differ significantly [2].
References	[1]. Iodine-lithium-alpha-dextrin (ILαD) against Staphylococcus aureus skin infections: a comparative study of in-vitro bactericidal activity and cytotoxicity between ILαD and povidone-iodine. J Hosp Infect. 2017 Jul 20. pii: S0195-6701(17)30395-X. [2]. Effect of the Hand Antiseptic Agents Benzalkonium Chloride, Povidone-Iodine, Ethanol, and Chlorhexidine Gluconate on Atopic Dermatitis in NC/Nga Mice. Int J Med Sci. 2015; 12(2): 116–125.
Additional Infomation	An iodinated polyvinyl polymer used as topical antiseptic in surgery and for skin and mucous membrane infections, also as aerosol. The iodine may be radiolabeled for research purposes. See also: Povidone-Iodine (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO : ~15 mg/mL H2O : ~2.4 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: 100 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7401 mL	13.7005 mL	27.4010 mL
	5 mM	0.5480 mL	2.7401 mL	5.4802 mL
	10 mM	0.2740 mL	1.3701 mL	2.7401 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.