Pociredir (FTX-6058) HCl is a potent, orally bioactive inhibitor of embryonic ectoderm development (EED). Pociredir HCl induces HbF protein expression in cellular and murine models. Pociredir HCl is indicated for the study of certain hemoglobinopathies such as sickle cell disease and beta-thalassemia.

Physicochemical Properties

Molecular Formula	C22H19CLFN5O2
Molecular Weight	439.87
Exact Mass	439.121
CAS #	2490676-19-0
Related CAS #	Pociredir;2490676-18-9
PubChem CID	165010517
Appearance	Light yellow to yellow solid powder
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	1
Heavy Atom Count	31
Complexity	628
Defined Atom Stereocenter Count	1
SMILES	C1(C2=CC3=C(NCC4C(F)=CC=C5C=4[C@@](CO3)([H])CO5)N3C=NN=C23)=C(N=CC=C1)C.Cl
InChi Key	JOYSKAVINOJMDD-ZOWNYOTGSA-N
InChi Code	InChI=1S/C22H18FN5O2.ClH/c1-12-14(3-2-6-24-12)15-7-19-22(28-11-26-27-21(15)28)25-8-16-17(23)4-5-18-20(16)13(9-29-18)10-30-19;/h2-7,11,13,25H,8-10H2,1H3;1H/t13-;/m0./s1
Chemical Name	(15S)-21-fluoro-10-(2-methylpyridin-3-yl)-13,17-dioxa-3,5,7,8-tetrazapentacyclo[13.6.1.04,12.05,9.018,22]docosa-1(21),4(12),6,8,10,18(22),19-heptaene;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Pociredir hydrochloride binds to EED to inhibit PRC2, hence inducing robust production of HbF protein in both mouse and cell models[2].
References	[1]. Boerner LK. Virtual meeting delivers first time drug structures. April 12, 2021. [2]. Fulcrum Therapeutics Presents Published Structure of Investigational Small Molecule FTX-6058 at the American Chemical Society (ACS) Spring 2021 Virtual Conference.

Solubility Data

Solubility (In Vitro)

DMSO : 125 mg/mL (284.17 mM) H2O : 2 mg/mL (4.55 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2734 mL	11.3670 mL	22.7340 mL
	5 mM	0.4547 mL	2.2734 mL	4.5468 mL
	10 mM	0.2273 mL	1.1367 mL	2.2734 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.