Pixatimod free acid (PG545 free acid) is a mimetic of Heparan Sulfate with heparanase inhibitory activity. Pixatimod free acid binds to the S1 receptor binding domain (RBD) of the SARS-CoV-2 virus and inhibits SARS-CoV-2 isolates with an EC50 of 2.4-13.8 µg/mL. Pixatimod free acid is an agonist of the TLR9 signaling pathway, which can enhance the activity of dendritic cells (DCs), activate natural killer cells, and has immunomodulatory and anti-tumor activities.

Physicochemical Properties

Molecular Formula	C51H88O60S13
Molecular Weight	2078.05
CAS #	1144617-49-1
SMILES	S(=O)(=O)(O)OC1C(C(C(COS(=O)(=O)O)OC1OC1CC[C@]2(C)[C@@H](C1)CC[C@H]1[C@@H]2CC[C@@]2(C)[C@@H](C(C)CCCC(C)C)CC[C@@H]21)OC1C(C(C(C(COS(=O)(=O)O)O1)OC1C(C(C(C(COS(=O)(=O)O)O1)OC1C(C(C(C(COS(=O)(=O)O)O1)OS(=O)(=O)O)OS(=O)(=O)O)OS(=O)(=O)O)OS(=O)(=O)O)OS(=O)(=O)O)OS(=O)(=O)O)OS(=O)(=O)O)OS(=O)(=O)O
Synonyms	PG545 free acid
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Synthetic heparan sulfate mimetic pixatimod (PG545) potently inhibits SARS-CoV-2 by disrupting the spike–ACE2 interactionJ. ACS central science, 2022, 8(5): 527-545. [2]. Phase Ib open-label, multicenter study of pixatimod, an activator of TLR9, in combination with nivolumab in subjects with microsatellite-stable metastatic colorectal cancer, metastatic pancreatic ductal adenocarcinoma and other solid tumors. J Immunother Cancer. 2023 Jan;11(1):e006136.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.4812 mL	2.4061 mL	4.8122 mL
	5 mM	0.0962 mL	0.4812 mL	0.9624 mL
	10 mM	0.0481 mL	0.2406 mL	0.4812 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.