Phosphatidylglycerol is a naturally occurring anionic phospholipid that is a component of plant, animal, and bacterial cell membranes. Phosphatidylglycerol is less abundant in prokaryotes and eukaryotes than phosphatidylethanolamine, and in eukaryotes less abundant than phosphatidylcholine. Phosphatidylglycerol is formed through the reaction between CDP-diglycerol and L-α-glycerol 3-phosphate followed by dephosphorylation, and is the metabolic precursor of cardiolipin. Phosphatidylglycerol containing polyunsaturated and monounsaturated fatty acyl chains inhibits and promotes the proliferation of murine keratinocytes, respectively. Phosphatidylglycerol is the second largest lipid component of mammalian lung surfactant, accounting for 10% of lipids, and levels are reduced in lung surfactant of infants with respiratory distress syndrome. Phosphatidylglycerol (egg) is a mixture of phosphatidylglycerols extracted from eggs, with various fatty acyl groups at the sn-1 and sn-2 positions.

Physicochemical Properties

Molecular Formula	C40H76NAO10P
Molecular Weight	770.98898601532
Exact Mass	268.032
CAS #	383907-64-0
PubChem CID	46891828
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	10
Rotatable Bond Count	41
Heavy Atom Count	52
Complexity	875
Defined Atom Stereocenter Count	1
SMILES	CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC(CO)O)OC(=O)CCCCCCC/C=C\CCCCCCCC.[Na+]
InChi Key	FJXDNGDRHUDFST-XQYKCTAGSA-M
InChi Code	InChI=1S/C40H77O10P.Na/c1-3-5-7-9-11-13-15-17-18-20-22-24-26-28-30-32-40(44)50-38(36-49-51(45,46)48-34-37(42)33-41)35-47-39(43)31-29-27-25-23-21-19-16-14-12-10-8-6-4-2;/h17-18,37-38,41-42H,3-16,19-36H2,1-2H3,(H,45,46);/q;+1/p-1/b18-17-;/t37?,38-;/m1./s1
Chemical Name	sodium;2,3-dihydroxypropyl [(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxypropyl] phosphate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Furse S. Is phosphatidylglycerol essential for terrestrial life?. Journal of chemical biology, 2017, 10(1): 1-9. [2]. Distinct effects of different phosphatidylglycerol species on mouse keratinocyte proliferation. PLoS One, 2014, 9(9): e107119.

Solubility Data

Solubility (In Vitro)

DMSO: 10 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.2970 mL	6.4852 mL	12.9703 mL
	5 mM	0.2594 mL	1.2970 mL	2.5941 mL
	10 mM	0.1297 mL	0.6485 mL	1.2970 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.