Palmitic acid-d3 is the deuterated form of Palmitic acid. Palmitic acid is a long-chain saturated fatty acid commonly found in animals and plants. Palmitic acid can induce the expression of glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP) in mouse granulosa cells.

Physicochemical Properties

Molecular Formula	C16H29D3O2
Molecular Weight	259.44
Exact Mass	256.24
CAS #	75736-53-7
Related CAS #	Palmitic acid;57-10-3;Palmitic acid sodium;408-35-5
PubChem CID	12358530
Appearance	White to off-white solid powder
Density	0.862 g/cm3 at 25 °C
Boiling Point	271.5 °C(lit.) at 100 mmHg
Melting Point	61-64 °C
LogP	5.552
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	14
Heavy Atom Count	18
Complexity	178
Defined Atom Stereocenter Count	0
SMILES	[2H]C([2H])([2H])CCCCCCCCCCCCCCC(=O)O
InChi Key	IPCSVZSSVZVIGE-FIBGUPNXSA-N
InChi Code	InChI=1S/C16H32O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16(17)18/h2-15H2,1H3,(H,17,18)/i1D3
Chemical Name	16,16,16-trideuteriohexadecanoic acid
Synonyms	palmitic acid d3
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
References	[1]. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216. [2]. Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res. 2002 Sep-Oct;22(5):2587-90.
Additional Infomation	Palmitic acid(d3) is a long-chain fatty acid and a deuterated fatty acid.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (385.45 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.64 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8545 mL	19.2723 mL	38.5446 mL
	5 mM	0.7709 mL	3.8545 mL	7.7089 mL
	10 mM	0.3854 mL	1.9272 mL	3.8545 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.