PROTAC CDK9 degrader-7 is a PROTAC that specifically targets CDK9. PROTAC CDK9 degrader-7 mediates CDK9 degradation through the proteasome.

Physicochemical Properties

Molecular Formula	C43H50CL2N8O9
Molecular Weight	893.81
CAS #	2935587-90-7
Appearance	Typically exists as solid at room temperature
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In Molm-13 cells, PROTAC CDK9 degrader-7 (compound 15f) degrades CDK9 with an IC50 of 40 nM, demonstrating maximal efficacy [1].
Cell Assay	Western Blot Analysis[1] Cell Types: MV411 cells Tested Concentrations: 0.01 μM, 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 5 μM Incubation Duration: 6 h Experimental Results: Dramatically diminished the protein level of MCL2, and almostly and competely degraded CKD9 at low concentration as 0.1 μM.
References	[1]. Tokarski RJ 2nd, et al. Bifunctional degraders of cyclin dependent kinase 9 (CDK9): Probing the relationship between linker length, properties, and selective protein degradation. Eur J Med Chem. 2023 Jun 5;254:115342.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~111.88 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.80 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1188 mL	5.5940 mL	11.1881 mL
	5 mM	0.2238 mL	1.1188 mL	2.2376 mL
	10 mM	0.1119 mL	0.5594 mL	1.1188 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.