PROTAC AR Degrader-4 contains IAP ligand binding group, linker and androgen receptor (AR) ligand binding group. cIAP1-based degraders are also known as SNIPERs.

Physicochemical Properties

Molecular Formula	C45H68F3N3O11
Molecular Weight	884.03
Related CAS #	PROTAC AR Degrader-4;1351169-31-7
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	cIAP1
ln Vitro	IAPs-dependent Protein Erasers that are Specific and Nongenetic (SNIPERs) are two-component compounds that are created by combining an IAPs recognition structure with a target protein recognition structure. Three processes are involved in directing protein breakdown. 1. SNIPER's two recognition structures enable the formation of a complex that binds the target protein to IAPs, which contain E3 ligase activity. IAPs cause the target protein to become polyubiquitinated. 3. Proteases breakdown the polyubiquitinated protein.
References	[1]. Design, synthesis and biological evaluation of nuclear receptor-degradation inducers. Bioorg Med Chem. 2011 Nov 15;19(22):6768-78.

Solubility Data

Solubility (In Vitro)

DMSO :~200 mg/mL (~226.24 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (5.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 5 mg/mL (5.66 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1312 mL	5.6559 mL	11.3118 mL
	5 mM	0.2262 mL	1.1312 mL	2.2624 mL
	10 mM	0.1131 mL	0.5656 mL	1.1312 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.