PPOH, a fatty acid analogue, is a selective cyclooxygenase (COX) inhibitor that can suppress arachidonic acid cyclooxygenase activity in renal cortical microsomes. In addition, the IC50s of PPOH on CYP4A2 and CYP4A3 are 22 μM and 6.5 μM, respectively. PPOH is a reagent for click chemistry. It contains Alkyne groups and could undergo CuAAc (copper-catalyzed azide-alkyne cycloaddition reaction) with compounds bearing an Azide group.

Physicochemical Properties

Molecular Formula	C15H18O3
Molecular Weight	246.30
Exact Mass	246.125
CAS #	206052-01-9
PubChem CID	53741002
Appearance	Light yellow to yellow solid powder
Density	1.1±0.1 g/cm3
Boiling Point	411.0±40.0 °C at 760 mmHg
Flash Point	151.1±20.8 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.534
LogP	3.05
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	8
Heavy Atom Count	18
Complexity	289
Defined Atom Stereocenter Count	0
InChi Key	CUNYTKVXYZYERK-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H18O3/c1-2-12-18-14-10-7-6-9-13(14)8-4-3-5-11-15(16)17/h1,6-7,9-10H,3-5,8,11-12H2,(H,16,17)
Chemical Name	6-(2-prop-2-ynoxyphenyl)hexanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	When a 30-minute NADPH pre-incubation is present, PPOH (5 μM) can suppress CYP2C9 activity by 63% and 90%, respectively, and by 63% when NADPH is not included in the 30-minute pre-incubation [1].
References	[1]. Effects of acetylenic epoxygenase inhibitors on recombinant cytochrome p450s. Drug Metab Dispos. 2011 Jul;39(7):1221-6.
Additional Infomation	PPOH is an aromatic ether.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (406.01 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.0601 mL	20.3004 mL	40.6009 mL
	5 mM	0.8120 mL	4.0601 mL	8.1202 mL
	10 mM	0.4060 mL	2.0300 mL	4.0601 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.