PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer and is widely used as biochemical compounds.

Physicochemical Properties

Molecular Formula	C8H18N2O6S2
Molecular Weight	302.37
Exact Mass	670.067
CAS #	5625-37-6
Related CAS #	PIPES disodium;76836-02-7;PIPES-d18;352534-95-3
PubChem CID	79723
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Melting Point	>300 °C(lit.)
Index of Refraction	1.566
LogP	-4.21
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	6
Heavy Atom Count	18
Complexity	397
Defined Atom Stereocenter Count	0
InChi Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
Chemical Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	173 g of 1,4-piperazinediethanesulfonic acid are dissolved in 1 L of deionized water to create the pH PIPES buffer. Pellets of sodium hydroxide are added to the PIPES buffer to bring its pH down to 6.8. Lipid loss and artifacts caused by glutaraldehyde fixation can be avoided by using PIPES buffer [1].
References	[1]. Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics. Technol Cancer Res Treat. 2017 Dec;16(6):997-1005.
Additional Infomation	2,2'-piperazine-1,4-diylbisethanesulfonic acid is a Good's buffer substance, pKa = 6.8 at 20 ℃. It is a conjugate acid of a 2,2'-piperazine-1,4-diylbisethanesulfonate.

Solubility Data

Solubility (In Vitro)	H2O: 5 mg/mL (16.54 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 2 mg/mL (6.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3072 mL	16.5360 mL	33.0721 mL
	5 mM	0.6614 mL	3.3072 mL	6.6144 mL
	10 mM	0.3307 mL	1.6536 mL	3.3072 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.