Tos-PEG3 is a PEG-based PROTAC (PROteolysis TArgeting Chimera) linker that can be used for PROTAC synthesis. Tos-PEG3 (Structure 1) may be utilized to prepare 3'-aminooxy oligonucleotide solid supports.

Physicochemical Properties

Molecular Formula	C13H20O6S
Molecular Weight	304.3593
Exact Mass	304.098
CAS #	77544-68-4
PubChem CID	10913735
Appearance	Typically exists as solid at room temperature
Density	1.234±0.06 g/cm3
Boiling Point	460.0±35.0 °C
LogP	1.806
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	10
Heavy Atom Count	20
Complexity	329
Defined Atom Stereocenter Count	0
SMILES	S(C1C([H])=C([H])C(C([H])([H])[H])=C([H])C=1[H])(=O)(=O)OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])OC([H])([H])C([H])([H])O[H]
InChi Key	RSUCRUOTLCQWFX-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H20O6S/c1-12-2-4-13(5-3-12)20(15,16)19-11-10-18-9-8-17-7-6-14/h2-5,14H,6-11H2,1H3
Chemical Name	2-[2-(2-hydroxyethoxy)ethoxy]ethyl 4-methylbenzenesulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. PROTACs target and selectively degrade target proteins by taking advantage of the intracellular ubiquitin-proteasome system.
References	[1]. Noël M, et al. Solid Supports for the Synthesis of 3'-Aminooxy Deoxy- or Ribo-oligonucleotides and Their 3'-Conjugation by Oxime Ligation.J Org Chem. 2019 Nov 15;84(22):14854-14860.

Solubility Data

Solubility (In Vitro)	Ethanol : ~100 mg/mL (~328.56 mM) DMSO : ~100 mg/mL (~328.56 mM) H2O : ~10 mg/mL (~32.86 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 16.67 mg/mL (54.77 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2856 mL	16.4279 mL	32.8558 mL
	5 mM	0.6571 mL	3.2856 mL	6.5712 mL
	10 mM	0.3286 mL	1.6428 mL	3.2856 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.