PARP1-IN-33 (Example 6) is a PARP1 inhibitor (IC50: 0.41 nM). PARP1-IN-33 has retinal cell protective effects with an EC50 of 0.02 nM (inhibits hydrogen peroxide-induced MTS activity in human retinal pigment epithelial cells).

Physicochemical Properties

Molecular Formula	C23H24CLFN4O
Molecular Weight	426.91
CAS #	2640677-68-3
Appearance	White to off-white solid powder
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	PARP1 0.41 nM (IC50)
ln Vitro	PARP1-IN-33 has a strong protective effect against retinal cell damage caused by hydrogen peroxide[1].
Cell Assay	Cell Viability Assay [1] Cell Types: H2O2 induced human retinal pigment epithelial Cell Types Tested Tested Concentrations: Less than 150 nM Incubation Duration: 6 h Experimental Results: Protected against hydrogen peroxide-induced damage to retinal cells.
References	[1]. Isoquinolinone derivative, preparation method therefor, and pharmaceutical composition, comprising same as active ingredient, for prevention or treatment of poly(adp-ribose)polymerase-1 (parp-1)-associated disease, WO2021086077, WPO, 2021-05-06.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3424 mL	11.7121 mL	23.4241 mL
	5 mM	0.4685 mL	2.3424 mL	4.6848 mL
	10 mM	0.2342 mL	1.1712 mL	2.3424 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.