Oxyntomodulin (bovine, porcine) is a 37-amino-acid peptide hormone that is a glucagon-like peptide 1 (GLP-1) receptor agonist (activator).

Physicochemical Properties

Molecular Formula	C192H295N59O60S
Molecular Weight	4421.86
CAS #	62340-29-8
Related CAS #	Oxyntomodulin (bovine, porcine) (TFA)
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

More effective at promoting weight loss than selective GLP1R agonists, oxytomodulin (bovine, porcine) is a peptide hormone released from the intestine during the postprandial state. It does this by activating the glucagon-like peptide-1 receptor (GLP1R) and glucagon receptor (GCGR). Enteroendocrine L cells are the main source of oxytomodulin, which is generated through the prohormone convertase 1/3 processing of proglucagon precursor. Although it has a lower affinity than GLP-1 and glucagon, oxytomodulin is a full agonist in cell lines overexpressing human GLP1R and GCGR-mediated cAMP accumulation [1].

References

[1]. Alessandro Pocai. Action and therapeutic potential of oxyntomodulin. Mol Metab. 2013 Dec 14;3(3):241-51.

Solubility Data

Solubility (In Vitro)

H2O: 50 mg/mL (11.31 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (0.57 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (0.57 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (0.57 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.2261 mL	1.1307 mL	2.2615 mL
	5 mM	0.0452 mL	0.2261 mL	0.4523 mL
	10 mM	0.0226 mL	0.1131 mL	0.2261 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.